Pensos 3D vivos e activos customizados para feridas crónicas

The project aims the development of customized WD by engineering 3D-printed multifunctional hydrogels for wound healing. Specifically, by joining the suitable properties of a regenerative hydrogel with the incorporation of live microalgae for oxygen production, healing will be assured, and then depending on each patient’s needs this regenerative platform will be customized with different therapeutic agent formulations (i.e. antimicrobial nanoparticles, debridement enzymes) assuring a therapeutic platform not prone to the development of resistance. i) The proposed research addresses the following important challenges: a) Complexity of chronic wound management b) Achievement of a healing WD platform suitable for different patients and wounds c) Achievement of different nano-delivery systems regarding different therapeutic needs and thus WD customization. d) Selection of antimicrobial agents not prone to the development of resistance (due to their mechanism of action). ii) The proposed objectives are beyond the state of the art due to: a) Use of emergent approaches (3D-printing, Artificial Intelligence tools, nanotechnology, biotech) to ensure the quality of the final product; b) Combination of the advantages of different eco-friendly medical grade polymers, antimicrobials not prone to the development of resistance (e.g., biosurfactants, antiseptics) and/or, debridement enzymes in nano-delivery systems in one single 3D-structure; b) Multi-functionalization of the 3D-hydrogel with regenerative and oxygen-producing components (hyaluronic acid and microalgae, respectively); c) Functionalize/load the 3D-regenerative platform with customized hydrogels loaded with the produced nanoparticles regarding the need for specific drugs and doses; d) Conduct a multidisciplinary approach to select the 3D-printed WD with an optimal regenerative, therapeutic activity and biosafety profile. e) Establish advanced in vitro test systems for 3D printed WDs to select the ones with a suitable regenerative, antibacterial, and biosafety profile; f) Gather a multidisciplinary team from different research areas, clinicians, and industry focusing on solving chronic wound challenges and implementing new innovative trends in chronic wound management. Overall, 3D-Alive&Active-WD represents a groundbreaking endeavor to revolutionize wound care by engineering of customized wound dressings. The project novelty lies in combining the unique attributes of regenerative hydrogels with live microalgae for oxygen generation, and antimicrobial nanoparticles not prone to induce resistance ensuring robust healing outcomes and therapeutic.